https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:46773 Wed 30 Nov 2022 13:14:41 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39149 n = 12), we developed an indirect ELISA to measure TPO-Ab levels from one 3-mm DBS punch. The sensitivity and specificity of our assay for DBS samples ranged from 91.7–100% and 98.2–98.8%, respectively, using a cut-off value of ≥ 26 IU/mL. Intra-assay reliability for duplicate quality control DBS punches was 5.2%, while inter-assay reliability ranged from 11.5–24.4% for high, medium, and low DBS controls. Dilutional linearity ranged from 80 to 120%, and spike and recovery experiments indicated that the DBS matrix does not interfere with the detection of TPO-Ab. TPO-Ab levels remained stable in DBS samples stored at − 28 °C or − 80 °C, but decreased over time in DBS samples kept at 22 °C or at 37 °C. Conclusions: We developed an in-house, kit-independent indirect ELISA assay to determine individuals’ TPO-Ab positivity based on dried blood spots, representing a cost-effective method with potential applications in a range of research settings.]]> Thu 19 May 2022 15:40:39 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:32041 Thu 09 Dec 2021 11:03:55 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45440 Fri 28 Oct 2022 12:58:16 AEDT ]]>